Experimental carcinogenesis on the oropharyngeal mucosa of rats with hydrochloric acid, sodium nitrate and pepsin

Acta Cirurgica Brasileira

André Del Negro²  Marina Raquel Araújo¹  Alfio José Tincani¹  Luciana Meirelles¹  Antônio Santos Martins¹  Nelson Adami Andreollo¹ 

[1],UNICAMP Department of Surgery Division of Head and Neck,Brazil

关键词: Gastroesophageal Reflux;    Hydrochloric Acid;    Nitrates;    Carcinoma;    Squamous Cell;    Rats;    Refluxo Gastroesofágico;    Ácido Clorídrico;    Nitratos;    Carcinoma de Células Escamosas;    Ratos;   

DOI  :  10.1590/S0102-86502008000400007

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the carcinogenic action of hydrochloric acid, pepsin and sodium nitrate on the oropharyngeal mucosa of rats, simulating the reflux of gastric contents. METHODS: Eighty-two Wistar rats were divided in seven groups and submitted to 2 or 3 weekly applications of hydrochloric acid, pepsin and sodium nitrate on the pharyngeal mucosa during six months. Study groups comprised 12 animals each. Rats in groups I and II were submitted to 2 (GI) or 3 (GII) weekly applications of 0.1N hydrochloric acid. Groups III and IV were submitted to 2 (GIII) or 3 (GIV) weekly applications of 0.1N hydrochloric acid solution with pepsin. Groups V and VI were submitted to 2 (GV) or 3 (GVI) weekly applications of 0.1N hydrochloric acid and treated with daily nitrate diluted in water. Group VII consisted of 10 animals submitted to 2 weekly applications of filtered water. RESULTS: No dysplasia, intra-epithelial neoplasia or invasive carcinomas were detected. Inflammatory changes were observed in varying degrees and mast cells were more common in Groups V and VI (p=0.006). CONCLUSION: The data of the current study could not corroborate the hypothesis that gastroesophageal and pharyngolaryngeal refluxes are carcinogenic factors to the laryngopharyngeal mucosa, and more studies are necessary in the future.

【 授权许可】

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