Acta Cirurgica Brasileira | |
Acute pulmonary injury induced by experimental muscle trauma | |
Márcia Andréa Da Silva Carvalho Sombra2  Marcelo Pinho Pessoa De Vasconcelos1  Sergio Botelho Guimarães1  Rodrigo Dornfeld Escalante1  José Huygens Parente Garcia1  Paulo Roberto Leitão De Vasconcelos1  | |
[1] ,UFC Department of SurgeryCeara ,Brazil | |
关键词: Models; Muscle; Acute Lung Injury; Dexamethasone; Peroxidase; Rats; Modelos Animais; Músculo Esquelético; Lesão Pulmonar Aguda; Dexametasona; Peroxidase; Ratos; | |
DOI : 10.1590/S0102-86502011000700009 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To develop an easily reproducible model of acute lung injury due to experimental muscle trauma in healthy rats. METHODS: Eighteen adult Wistar rats were randomized in 3 groups (n=6): G-1- control, G-2 - saline+trauma and G-3 - dexamethasone+trauma. Groups G-1 and G-2 were treated with saline 2,0ml i.p; G-3 rats were treated with dexamethasone (DE) (2 mg/kg body weight i.p.). Saline and DE were applied 2h before trauma and 12h later. Trauma was induced in G-2 and G-3 anesthetized (tribromoethanol 97% 100 ml/kg i.p.) rats by sharp section of anterior thigh muscles just above the knee, preserving major vessels and nerves. Tissue samples (lung) were collected for myeloperoxidase (MPO) assay and histopathological evaluation. RESULTS: Twenty-four hours after muscle injury there was a significant increase in lung neutrophil infiltration, myeloperoxidase activity and edema, all reversed by dexamethasone in G-3. CONCLUSION: Trauma by severance of thigh muscles in healthy rats is a simple and efficient model to induce distant lung lesions.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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