Acute pulmonary injury induced by experimental muscle trauma

Acta Cirurgica Brasileira

Márcia Andréa Da Silva Carvalho Sombra²  Marcelo Pinho Pessoa De Vasconcelos¹  Sergio Botelho Guimarães¹  Rodrigo Dornfeld Escalante¹  José Huygens Parente Garcia¹  Paulo Roberto Leitão De Vasconcelos¹ 

[1] ,UFC Department of SurgeryCeara ,Brazil

关键词: Models;    Muscle;    Acute Lung Injury;    Dexamethasone;    Peroxidase;    Rats;    Modelos Animais;    Músculo Esquelético;    Lesão Pulmonar Aguda;    Dexametasona;    Peroxidase;    Ratos;   

DOI  :  10.1590/S0102-86502011000700009

来源: SciELO

【 摘 要 】

PURPOSE: To develop an easily reproducible model of acute lung injury due to experimental muscle trauma in healthy rats. METHODS: Eighteen adult Wistar rats were randomized in 3 groups (n=6): G-1- control, G-2 - saline+trauma and G-3 - dexamethasone+trauma. Groups G-1 and G-2 were treated with saline 2,0ml i.p; G-3 rats were treated with dexamethasone (DE) (2 mg/kg body weight i.p.). Saline and DE were applied 2h before trauma and 12h later. Trauma was induced in G-2 and G-3 anesthetized (tribromoethanol 97% 100 ml/kg i.p.) rats by sharp section of anterior thigh muscles just above the knee, preserving major vessels and nerves. Tissue samples (lung) were collected for myeloperoxidase (MPO) assay and histopathological evaluation. RESULTS: Twenty-four hours after muscle injury there was a significant increase in lung neutrophil infiltration, myeloperoxidase activity and edema, all reversed by dexamethasone in G-3. CONCLUSION: Trauma by severance of thigh muscles in healthy rats is a simple and efficient model to induce distant lung lesions.

【 授权许可】

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