The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats

Acta Cirurgica Brasileira

Luiz Antonio Maksoud Bussuan¹  Djalma José Fagundes²  Guido Marks¹  Priscila Maksoud Bussuan¹  Roberto Teruya¹ 

[1] ,UNIFESP Department of SurgerySP ,Brazil

关键词: Fas Ligand Protein;    Azoxymethane;    Oxidative Stress;    Apoptosis;    Rats;    Proteína Ligante Fas;    Azoximetano;    Estresse Oxidativo;    Apoptose;    Ratos;   

DOI  :  10.1590/S0102-86502010000600008

来源: SciELO

【 摘 要 】

PURPOSE: To study the protein Fas ligand (FasL) on the expression of apoptosis, using a model of oxidative stress induced by azoxymethane (AOM), in the crypt of colon in rats. METHODS: Wistar rats (n=14) were assigned into two groups: control (n=7) and AOM (n=7). A single subcutaneous administration of AOM (5mg/kg) or saline solution was performed at the beginning of third week and after three hours samples of proximal colon were collected. The expression of FasL was quantified (Software ImageLab) in percentage of areas in the top, base and all crypt. Results were expressed as mean ± sd (Shapiro-Wilks test and t Student test) (p < 0.05). RESULTS: In the animals of CG there was no significant difference between the FasL expression of the top (10.75±3.33) and basal (11.14±3.53) colon crypt (p=0.34293740). In the animals of AOM there was no significant difference between the FasL expression of the top (8.86±4.19) and basal (8.99±4.08) colon crypt (p=0.78486003). In the animals of CG (10.95±3.43) and AOM (8.92±4.13) there was a significant difference of the FasL expression (p=0.026466821). A significantly decrease on the FasL expression was observed in the animals of CG (10.75±3.33) and AOM (8.86±4.19) in the top crypt (p=0.00003755*). A significant decrease was also observed in the animals of CG (11.14±3.53) and AOM (8.99±4.08) in the basal colon crypt (p=0.00000381**). CONCLUSION: Azoxymethane induce the oxidative stress and the significantly decrease of FasL expression, although there is no significant difference between basal and top of colon crypt linked to consumption-activation of Fas ligand.

【 授权许可】

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