Expression of FAS ligand in the ipsilateral and contralateral testicles of rats subjected to the torsion of the unilateral testicular cord

Acta Cirurgica Brasileira

Peterson Vieira De Assis¹  Ricardo Dutra Aydos²  Iandara Schettert Silva²  Guido Marks²  Luis Carlos Takita²  Marco Antonio Gonçalves¹  Rondon Tosta Ramalho¹ 

[1] ,UFMS Department of Surgery Campo Grande MS ,Brazil

关键词: Fas Ligand Protein;    Spermatic Cord Torsion;    Immunohistochemistry;    Ischemia;    Reperfusion Injury;    Oxidative Stress;    Rats;   

DOI  :  10.1590/S0102-86502013000700007

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the expression of FAS ligand (FASL) in ipsilateral and contralateral testicles of rats submitted to ischemia/reperfusion. METHODS: Wistar rats (n=21) distributed into groups control (GC), n=5, testicular exposure; ischemia (GI), (n=8), Torsion in the left testicular Cord (TCT) for three hours followed by orchiectomy without distortion and orchietomy of the contralateral testicle after 24 hours; and reperfusion (GR), (n=8), left TCT for 3 hours and distortion and repositioning on the scrotum and bilateral orchiectomy after 24 hours. Quantification of the FASL expression by immune-histochemistry. RESULTS: Statistical analysis showed similarity between GC and GI (p>0.05), differences detected are concentrated on the GR (p<0.05), increase in immunoexpression of FASL in the subgroups Right GR (406.8+-61.5) and Left GR (135.3 +-28.9) with significant predominance in the GR subgroup. CONCLUSION: Ischemia/reperfusion increased the FASL expression significantly in contralateral testicles in GR, in rats.

【 授权许可】

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