Ternatin pretreatment attenuates testicular injury induced by torsion/detorsion in Wistar rats

Acta Cirurgica Brasileira

Sergio Botelho Guimarães²  Jefferson Menezes Viana Santos¹  Alan Arruda Aragão¹  Osamu Sandes Kimura¹  Edilberto R Silveira¹  Paulo Roberto Leitão De Vasconcelos¹ 

[1] ,UFC Department of Surgery LABCEX Ceara ,Brazil

关键词: Flavonoids;    Spermatic Cord Torsion;    Ischemia;    Reperfusion;    Rats;    Flavonóides;    Torção do Cordão Espermático;    Isquemia;    Reperfusão;    Ratos;   

DOI  :  10.1590/S0102-86502011000400014

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the possible protective role of the bioflavonoid ternatin (TTN) when administered before induction of ischemia/reperfusion injury in rat testis. METHODS: Thirty-six Wistar rats were randomly assigned to 3 groups (n=12), divided in 2 subgroups (n=6). Saline 2.0ml (G-1), dimethylsulfoxide (DMSO) 3% solution (G-2) or TTN 12 mg/kg/dose (G-3) was administered ip. to all rats, respectively, 21, 12 and 1 hour before torsion. Anesthetized rats were subjected to ischemia (3 hours) induced by 720º torsion of the spermatic cord. Right testis and arterial blood samples were collected at the end of ischemia (T-0), and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). RESULTS: MDA decreased significantly (p<0,001) in G-2 and G-3 in T-0 and T-3 timepoints. Additional decrease in MDA was seen in G-3 after 3 hours of reperfusion (T-3). GSH increased significantly in G-2 (p<0.001) and G-3 (p<0.05) at the end the ischemia (T-0). A significant increase in GSH was seen 3 hours after testis detorsion (T-3) in G-2 rats. TAP values remained unchanged. CONCLUSION: The data provides in vivo evidence of the antiperoxidative and antioxidative properties of TTN in torted rat testis.

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