Acta Cirurgica Brasileira | |
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats | |
Idália Maria Brasil Burlamaqui2  Conceição Aparecida Dornelas1  Rodrigo Dornenfeld Escalante1  Daniel Magalhães Coutinho Mota1  Francisco José Cabral Mesquita1  Eduardo Rebouças Carvalho1  Lara Burlamaqui Veras1  Lusmar Veras Rodrigues1  | |
[1] ,UFC Postgraduate Program in Surgery Ceará,Brazil | |
关键词: Precancerous Conditions; Colorectal Neoplasms; Azoxymethane; Rats; Lesões Pré-Cancerosas; Neoplasias Colorretais; Azoximetano; Ratos; | |
DOI : 10.1590/S0102-86502010000200005 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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