Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats

Acta Cirurgica Brasileira

Idália Maria Brasil Burlamaqui²  Conceição Aparecida Dornelas¹  Rodrigo Dornenfeld Escalante¹  Daniel Magalhães Coutinho Mota¹  Francisco José Cabral Mesquita¹  Eduardo Rebouças Carvalho¹  Lara Burlamaqui Veras¹  Lusmar Veras Rodrigues¹ 

[1] ,UFC Postgraduate Program in Surgery Ceará,Brazil

关键词: Precancerous Conditions;    Colorectal Neoplasms;    Azoxymethane;    Rats;    Lesões Pré-Cancerosas;    Neoplasias Colorretais;    Azoximetano;    Ratos;   

DOI  :  10.1590/S0102-86502010000200005

来源: SciELO

【 摘 要 】

PURPOSE: Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. METHODS: Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. RESULTS: When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. CONCLUSION: Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License