Lung apoptosis after intra-pulmonary instillation of Benzo(a)pyrene in Wistar rats

Acta Cirurgica Brasileira

Baldomero Antonio Kato Da Silva²  Ricardo Dutra Aydos¹  Iandara Schettert Silva¹  Daniel Martins Pereira¹  Paulo De Tarso Camillo De Carvalho¹  Doroty Mesquita Dourado¹  Filipe Abdalla Dos Reis²  Renato Silva Nacer¹ 

[1] ,UFMS Central West Region

关键词: Apoptosis;    Benzo(a)pyrene;    Lung Neoplasms;    Carcinogens;    Rats;    Apoptose;    Benzo(a)pireno;    Neoplasias Pulmonares;    Carcinógenos;    Ratos;   

DOI  :  10.1590/S0102-86502010000100023

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the influence of pulmonary instillation of Benzo[a]pyrene in lung apoptosis of Wistar rats. METHODS: Male Rattus norvegicus albinus, Wistar lineage was carried through an intra-pulmonary instillation of the Benzo[a]pyrene (B[a]P) dilution in alcohol 70%. Three experimental groups had been formed with 08 animals each: Control Group (Alcohol 70%); B[a]P Group 40 mg/kg; e B[a]P Group 80mg/kg, submitted to euthanasia 16 and 18 weeks after the experimental procedure. The pulmonary sections had been processed by TUNEL method and submitted to the histomorphometric analysis to quantify the apoptotic cell number. RESULTS: After 16 weeks, mean of apoptotic cells number in control group (19,3±3,2) was greater than 40mg/Kg group (11,8±1,9; p<0,01) and 80mg/Kg group (7,0±1,4; p<0,01). Significant difference also observed between 40mg/Kg and 80mg/Kg (p<0,05). After 18 weeks, mean of apoptotic cells number in control group (18,0±2,2) was greater than 40mg/Kg group (8,8±1,7; p<0,01) and 80mg/Kg group (5,5±1,3; p<0,01). Significant difference wasn't observed between 40mg/Kg and 80mg/Kg (ns). CONCLUSION: Intra-pulmonary instillation of Benzo[a]pyrene induces significant decrease of apoptotic activity in lung tissue.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License