Development of a lethal model of peritonitis for assessment of laparoscopic and laparotomic treatments in rats

Acta Cirurgica Brasileira

Wilson Salgado Júnior²  José Sebastião Dos Santos²  Fernando De Queiroz Cunha¹ 

[1] ,USP FMRP Department of Surgery and Anatomy,Brazil

关键词: Peritonitis;    Laparotomy;    Laparoscopy;    Cytokines;    Lethality;    Rats;    Peritonite;    Laparotomia;    Laparoscopia;    Citocinas;    Letalidade;    Ratos;   

DOI  :  10.1590/S0102-86502007000100007

来源: SciELO

【 摘 要 】

PURPOSE: Development of a lethal model of peritonitis to assess the results of treating that peritonitis using videolaparoscopy and laparotomy. METHODS: We developed a model of peritonitis in rats using cecal ligation (CLP) against a 2-mm diameter rigid mold and puncture. Two experiments were performed: determination of seven-day lethality; and analysis of white cell counts, blood cultures and cytokines (Interleukin-1 beta, Tumor Necrosis Factor-alpha and IL-6). The animals were divided into four groups: I - Sham surgery; II - CLP; III - CLP + Videolaparoscopy; and IV- CLP + Laparotomy . RESULTS: Seven-day lethality was 0% in group I, 80% in the group II (p<0.05), 60% in group III , and 20 % in group IV. There was a significant reduction in leukocyte counts and higher levels of serum IL-1 beta, TNF-alpha and IL-6 in the group II compared to controls. The percentages of positive blood cultures were higher after videolaparoscopic compared to laparotomic treatment. CONCLUSION: The experimental model provoked a lethal form of peritonitis and that videolaparoscopic treatment had more bacteraemia than laparotomy.

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