Oxidative stress induced by torsion of the spermatic cord in young rats

Acta Cirurgica Brasileira

Sergio Botelho Guimarães²  Alan Arruda Aragão¹  Jefferson Menezes Viana Santos¹  Osamu De Sandes Kimura¹  Paulo Hudson Uchoa Barbosa¹  Paulo Roberto Leitão De Vasconcelos¹ 

[1] ,UFC Head of LABCEX Department of Surgery,Brazil

关键词: Antioxidants;    Testis;    Oxidative stress;    Malonaldehyde;    Glutathione;    Rats;    Antioxidantes;    Testículo;    Estresse oxidativo;    Malonaldeído;    Glutationa;    Ratos;   

DOI  :  10.1590/S0102-86502007000100005

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies. METHODS: Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP). RESULTS: Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. CONCLUSION: Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/reperfusion injury in young rat testis.

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