Experimental model for low level laser therapy on ischemic random skin flap in rats

Acta Cirurgica Brasileira

Rodrigo Paschoal Prado²  Richard Eloin Liebano²  Bernardo Hochman²  Carlos Eduardo Pinfildi²  Lydia Masako Ferreira¹ 

[1] ,UNIFESP,Brazil

关键词: Laser Therapy;    Low-Level;    Surgical Flaps;    Necrosis;    Rats;    Terapia a Laser de Baixa Intensidade;    Retalhos Cirúrgicos;    Necrose;    Ratos;   

DOI  :  10.1590/S0102-86502006000400013

来源: SciELO

【 摘 要 】

PURPOSE: To develop an experimental model to be used in the study of low level Laser therapy on viability of random skin flap in rats. METHODS: The sample was 24 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic sheet was interposed between the flap and donor site. Group 1 (control) underwent sham irradiation with diode laser (830 nm). Group 2 was submitted to laser irradiation with diode laser (830 nm). The animals were submitted to Laser therapy with 36 J/cm² energy density (72 seconds) immediately after the surgery and on the four subsequent days. The probe was usually held in contact with the skin flap surface on a point at 2.5 cm cranial from the flap base. On the seventh postoperative day, the percentage of necrotic area was measured and calculated. RESULTS: Group 1 reached an average necrotic area of 48.86%, Group 2 - 23.14%. After the statistic analysis, compared with the control group, Group 2 showed a statistically significant increase in survival area (p<0.001). CONCLUSION: The experimental model proved to be reliable to be used in the study of effects of low level laser therapy in random skin flap in rats.

【 授权许可】

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