Effect of 650 nm low-power laser on bone morphogenetic protein in bone defects induced in rat femors

Acta Cirurgica Brasileira

Paulo De Tarso Camillo De Carvalho²  Iandara Schettert Silva¹  Filipe Abdalla Dos Reis¹  Ana Carulina Guimarães Belchior¹  Gilberto Gonçalves Facco¹  Ronny Nascimento Guimarães¹  Guilherme Henrique Cardoso Fernandes¹  Amanda Silveira Denadai¹ 

[1],UNIDERP Department of Physiotherapy ,Brazil

关键词: Laser Therapy;    Low-Level;    Bone remodeling;    Bone morphogenetic protein;    Terapia a Laser de Baixa Potência;    Remodelação Óssea;    Proteínas Morfogenéticas Ósseas;   

DOI  :  10.1590/S0102-86502006001000014

来源: SciELO

【 摘 要 】

PURPOSE:To investigate the influence of 650 nm GaAlAs laser on the action of bone morphogenetic protein (BMP) in bone defects produced in rat femurs. METHODS: The sample consisted of 12 male albino Wistar rats (Rattus norvegicus). The animals were randomly divided into four experimental groups. After undergoing anesthesia, the fur was removed from the lateral face of the right thigh and surgical dissection was performed to view the femur region. A bone defect was created using a spherical diamond-tipped drill bit. In groups 1 and 2, the defect was filled with a paste of Gen-Tech bone-inducing substance. The animals were treated with GaAlAs laser, at a predetermined dose of joules/cm² for 80 seconds, over an area of 1 cm². Groups 2 and 4 were used as controls. Bone samples were removed to perform histological procedures and morphometric analyses on the 7th, 14th and 21st days after the operation. The results obtained were subjected to statistical analysis using ANOVA variance according to two criteria, with four repetitions, followed by the post hoc t test. The rejection level for the nullity hypothesis was 0.05 or 5% (alpha <= 0.05). RESULTS: In comparisons between G1, G2, G3 and G4, p = 0.024 was observed. In statistical comparisons using the t test for paired samples, only G1 vs. G4 presented a statistically significant result (p = 0.021). CONCLUSION: The association of low-power laser application and Gen-Tech bone-inducing substance achieved a better result than laser application alone or BMP use alone.

【 授权许可】

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