Effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing: experimental study in rats

Acta Cirurgica Brasileira

Antônio Carlos Perez²  Delcio Matos¹  Edna Frasson De Souza Montero¹  Heitor Soares De Souza¹  Fábio Colagrossi Paes Barbosa¹  Fabio Saad¹  Nelson Dip¹ 

[1],Federal University of Mato Grosso do Sul

关键词: Carboxymethylcellulose;    Colon;    Healing;    Rats;    Carboximetilcelulose;    Cólon;    Cicatrização;    Ratos;   

DOI  :  10.1590/S0102-86502005000100004

来源: SciELO

【 摘 要 】

PURPOSE: To analyze the effects of sodium hyaluronate and carboxymethylcellulose membrane on collagen and fibroblast formation in bowel suture healing in rats. METHODS: 48 male Wistar rats, weighing 250 to 343g, were randomized into two groups: group I - bowel suture without applying a biologically absorbable membrane and group II - bowel suture with application of an absorbable membrane. The two groups were divided into subgroups of 3, 14 and 30 days of observation, with 8 rats in each subgroup. All were sacrificed after the end of the observation period. RESULTS: No morbidity or mortality was observed during the experiment. The amounts of collagen in group I were 23.4%, 72.1% and 67.6% and in group II were 22.5%, 52.5% and 51.6%, for the subgroups of 3, 14 and 30 days, respectively. Comparison between groups showed that the 14-day (p=0.0013) and 30-day (p=0.0587) subgroups had significant variance, with larger collagen zones in animals in which the membrane was not applied. However, with regard to fibroblasts, group I had 2%, 13% and 8% and group II had 2%, 10% and 8%, for the 3-day (p=1.0), 14-day (p=0.3184) and 30-day (p=0.5995) subgroups, respectively, showing no significant variance. CONCLUSION: The use of the biologically absorbable membrane cause a decrease in collagen formation, while not altering the number of fibroblasts, in bowel suture healing in rats, without increased morbidity and mortality.

【 授权许可】

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