Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

Food Science and Technology (Campinas)

Raquel Duarte Da Costa Cunha Bandeira²  Thaís Matsue Uekane²  Carolina Passos Da Cunha²  Valnei Smarçaro Da Cunha²  Janaína Marques Rodrigues²  Ronoel Luiz De Oliveira Godoy¹  Marcus Henrique Campino De La Cruz¹ 

[1],National Institute of Metrology, Standardization and Industrial Quality - INMETRODuque de Caxias RJ ,Brazil

关键词: coffee;    mycotoxin;    validation;    HPLC;    MS;    café;    micotoxinas;    validação;    CLAE;    EM;   

DOI  :  10.1590/S0101-20612012005000120

来源: SciELO

【 摘 要 】

A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g-1. Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g-1 and 3.0 ng.g-¹, respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.

【 授权许可】

CC BY   
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