Weak phenotypic reversion of ivermectin resistance in a field resistant isolate of Haemonchus contortus by verapamil

Pesquisa Veterinária Brasileira

Fernando A. Borges²  Julhiano B. Rossini¹  Patrícia P. Velludo²  Carolina Buzzulini²  Gustavo H. Costa²  Marcelo B. Molento¹  Alvimar J. Costa¹ 

[1] ,Universidade Federal de Mato Grosso do Sul Faculdade de Medicina Veterinária e Zootecnia

关键词: Haemonchus contortus;    ivermectin;    P-glycoprotein;    resistance;    reversion;    verapamil;    Haemonchus contortus;    ivermectina;    Pglycoprotein;    resistance;    reversion;    verapamil;   

DOI  :  10.1590/S0100-736X2011000900002

来源: SciELO

【 摘 要 】

Recent advances in anthelmintic resistant phenotype reversion by Pgp modulating drugs in ruminant nematodes indicate that this can be a useful tool to helminth control. The aim of the present study was to evaluate the efficacy of ivermectin (IVM) in combination with verapamil (VRP), in oil or water-based vehicle, against an IVM-resistant field isolate of Haemonchus contortus through a larval migration assay and experimental infection trial. In the in vitro assay was observed a phenotypic reversion of H. contortus resistance to ivermectin at a high concentration of VRP, increasing IVM efficacy from 53.1% to 94.3. In the in vivo trial, IVM + VRP demonstrated 36.02% efficacy compared to the 7.75% of IVM alone. The vehicle formulation showed no influence in efficacy. These are the first results demonstrating the effect of VRP as a partial IVM-resistance phenotype reverser in a field isolate of IVM-resistant H. contortus experimentally inoculated in sheep.

【 授权许可】

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