Determinação simultânea de carbamazepina, fenitoína e fenobarbital em sangue seco em papel por cromatografia líquida de alta eficiência

Química Nova

Gabriela Martins Silva De Lima¹  Roberta Zilles Hahn¹  Cristina Rama¹  Liliane Rhoden¹  Paulina Hidalgo¹  Cleber Álvares Da Silva¹  Marina Venzon Antunes¹  Rafael Linden¹ 

[1] ,Universidade Feevale Instituto de Ciências da Saúde Novo Hamburgo RS ,Brasil

关键词: antiepileptic drugs;    dried blood spots;    HPLC;    therapeutic drug monitoring;   

DOI  :  10.5935/0100-4042.20140166

来源: SciELO

【 摘 要 】

Carbamazepine, phenobarbital and phenytoin were determined in dried blood spots (DBS) by high performance liquid chromatography, after extraction of 8 mm DBS using a mixture of acetonitrile and methanol. Analytes were separated by reversed-phase chromatography, with a run time of 17 minutes. Intra-assay and inter-assay precisions were in the 5.3 to 8.4% and 3.3 to 5.2% ranges, respectively. Accuracy was in the 98.8 to 104.3% range. The method had sensitivity to detect all analytes at levels below minimum therapeutic concentrations. The analytes were stable at 4 ºC and room temperature for up to 12 days and at 45 ºC for 9 days. The method was applied to 14 paired clinical samples of blood serum and DBS.

【 授权许可】

CC BY-NC   
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