Simultaneous determination of carbamazepine, phenytoin and phenobarbital in dried blood spots by high performance liquid chromatography

Química Nova

Lima, Gabriela Martins Silva de¹  Linden, Rafael¹  Hidalgo, Paulina¹  Rhoden, Liliane¹  Hahn, Roberta Zilles¹  Antunes, Marina Venzon¹  Silva, Cleber Álvares da¹  Universidade Feevale, Novo Hamburgo, Brasil¹  Rama, Cristina¹ 

关键词: antiepileptic drugs;    dried blood spots;    HPLC;    therapeutic drug monitoring.;   

DOI  :  10.5935/0100-4042.20140166

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Carbamazepine, phenobarbital and phenytoin were determined in dried blood spots (DBS) by high performance liquid chromatography, after extraction of 8 mm DBS using a mixture of acetonitrile and methanol. Analytes were separated by reversed-phase chromatography, with a run time of 17 minutes. Intra-assay and inter-assay precisions were in the 5.3 to 8.4% and 3.3 to 5.2% ranges, respectively. Accuracy was in the 98.8 to 104.3% range. The method had sensitivity to detect all analytes at levels below minimum therapeutic concentrations. The analytes were stable at 4 ºC and room temperature for up to 12 days and at 45 ºC for 9 days. The method was applied to 14 paired clinical samples of blood serum and DBS.

【 授权许可】

Unknown