Enzyme loading dependence of cellulose hydrolysis of sugarcane bagasse

Química Nova

Carlos Martín²  George Jackson De Moraes Rocha¹  Julliana Ribeiro Alves Dos Santos¹  Maria Carolina De Albuquerque Wanderley¹  Ester Ribeiro Gouveia¹ 

[1] ,University of Matanzas Department of Chemistry and Chemical Engineering ,Cuba

关键词: sugarcane bagasse;    enzymatic hydrolysis;    delignification;   

DOI  :  10.1590/S0100-40422012001000007

来源: SciELO

【 摘 要 】

The enzymatic hydrolysis of steam-pretreated sugarcane bagasse, either delignified or non-delignified, was studied as a function of enzyme loading. Hydrolysis experiments were carried out using five enzyme loadings (2.5 to 20 FPU/g cellulose) and the concentration of solids was 2% for both materials. Alkaline delignification improved cellulose hydrolysis by increasing surface area. For both materials, glucose concentrations increased with enzyme loading. On the other hand, enzyme loadings higher than 15 FPU/g did not result in any increase in the initial rate, since the excess of enzyme adsorbed onto the substrate restricted the diffusion process through the structure.

【 授权许可】

CC BY-NC   
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