Enzyme loading dependence of cellulose hydrolysis of sugarcane bagasse

Química Nova

Wanderley, Maria Carolina de Albuquerque¹  Rocha, George Jackson de Moraes¹  Santos, Julliana Ribeiro Alves dos¹  Gouveia, Ester Ribeiro¹  Universidade Federal de Pernambuco, Recife, Brasil¹  Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Campinas, Brasil¹  University of Matanzas, Cuba¹  Martín, Carlos¹  Institute for Wood Technology and Wood Biology, Hamburg, Germany¹ 

关键词: sugarcane bagasse;    enzymatic hydrolysis;    delignification.;   

DOI  :  10.1590/S0100-40422012001000007

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The enzymatic hydrolysis of steam-pretreated sugarcane bagasse, either delignified or non-delignified, was studied as a function of enzyme loading. Hydrolysis experiments were carried out using five enzyme loadings (2.5 to 20 FPU/g cellulose) and the concentration of solids was 2% for both materials. Alkaline delignification improved cellulose hydrolysis by increasing surface area. For both materials, glucose concentrations increased with enzyme loading. On the other hand, enzyme loadings higher than 15 FPU/g did not result in any increase in the initial rate, since the excess of enzyme adsorbed onto the substrate restricted the diffusion process through the structure.

【 授权许可】

Unknown