Enzyme loading dependence of cellulose hydrolysis of sugarcane bagasse

Quimica nova

Rocha, George Jackson de Moraes¹  Santos, Julliana Ribeiro Alves dos²  Wanderley, Maria Carolina de Albuquerque³  Gouveia, Ester Ribeiro³  Martín, Carlos⁴ 

[1] Institute for Wood Technology and Wood Biology, Hamburg, Germany;Laboratório Nacional de Ciência e Tecnologia do Bioetanol, Campinas, Brasil;Universidade Federal de Pernambuco, Recife, Brasil;University of Matanzas, Cuba

关键词: sugarcane bagasse;    enzymatic hydrolysis;    delignification. ;     ;    INTRODUCTION;   

DOI  :  10.1590/S0100-40422012001000007

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The enzymatic hydrolysisof steam-pretreated sugarcane bagasse, either delignified or non-delignified,was studied as a function of enzyme loading. Hydrolysis experiments were carriedout using five enzyme loadings (2.5 to 20 FPU/g cellulose) and the concentrationof solids was 2% for both materials. Alkaline delignification improved cellulosehydrolysis by increasing surface area. For both materials, glucose concentrationsincreased with enzyme loading. On the other hand, enzyme loadings higher than15 FPU/g did not result in any increase in the initial rate, since the excessof enzyme adsorbed onto the substrate restricted the diffusion process throughthe structure.

【 授权许可】

CC BY