The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover

Polymers

Ali Hamdy¹  Hesham Hamad²  Sara Abd Elhafez²  Rehab Ali² 

[1] Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technology Application (SRTA-City), New Borg El-Arab City P.O. Box 21934, Alexandria, Egypt;Fabrication Technology Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technology Application (SRTA-City), New Borg El-Arab City P.O. Box 21934, Alexandria, Egypt;

关键词: delignification;    lignocellulose;    ultrasonic irradiation;    autoclaving;    response surface methodology;    enzymatic hydrolysis;   

DOI  :  10.3390/polym13213762

来源: DOAJ

【 摘 要 】

Bio-based treatment technologies are gaining great interest worldwide, and significant efforts are being afforded to develop technology for the use of lignocellulosic biomass. The potential of corn stover (CS) as a feedstock for bioethanol production was investigated by creating an optimal pretreatment condition to maximize glucose production. The current study undertook the impact of novel physico-chemical pretreatment methods of CS, i.e., autoclave-assisted oxalate (CSOA) and ultrasound-assisted oxalate (CSOU), on the chemical composition of CS and subsequent saccharification and fermentation for bioethanol production. The delignification was monitored by physicochemical characterizations such as SEM, XRD, FTIR, CHNs, and TGA. The results evidenced that delignification and enzymatic saccharification of the CS pretreated by CSOA was higher than CSOU. The optimum enzymatic saccharification operating conditions were 1:30 g solid substrate/mL sodium acetate buffer at 50 °C, shaking speed 100 rpm, and 0.4 g enzyme dosage. This condition was applied to produce glucose from CS, followed by bioethanol production by S. cerevisiae using an anaerobic fermentation process after 72 h. S. cerevisiae showed high conversion efficiency by producing a 360 mg/dL bioethanol yield, which is considered 94.11% of the theoretical ethanol yield. Furthermore, this research provides a potential path for waste material beneficiation, such as through utilizing CS.

【 授权许可】

Unknown