Development and validation of a High Performance Liquid Chromatographic method for determination of etoposide in biodegradable polymeric implants

Química Nova

Ana Gabriela Reis Solano²  Gisele Rodrigues Da Silva¹  Silvia Ligório Fialho¹  Armando Da Silva Cunha Júnior¹  Gérson Antônio Pianetti¹ 

[1] ,Universidade Federal de São João Del Rei Faculdade de Farmácia Divinópolis MG ,Brasil

关键词: validation;    etoposide;    poly(ε-caprolactone);    intraocular implant;   

DOI  :  10.1590/S0100-40422012000600032

来源: SciELO

【 摘 要 】

A method using HPLC-UV was developed and validated for the determination of etoposide incorporated into polycaprolactone implants. The method was carried out in isocratic mode using a C18 column (250 x 4.6 mm; 5 µm), at 25 ºC, with acetonitrile and acetic acid 4% (70:30) as mobile phase, a flow rate of 2 mL/min, and UV detection at 285 nm. The method was linear (r² > 0.99) over the range of 5 to 65 µg/mL, precise (RSD < 5%), accurate (recovery of 98.7%), robust, selective regarding excipient of the sample, and had a quantitation limit equal to 1.76 µg/mL. The validated method can be successfully employed for routine quality control analyses.

【 授权许可】

CC BY-NC   
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