Development and validation of a High Performance Liquid Chromatographic method for determination of etoposide in biodegradable polymeric implants

Quimica nova

Fialho, Silvia Ligório¹  Pianetti, Gérson Antônio²  Cunha Júnior, Armando da Silva²  Silva, Gisele Rodrigues da³  Solano, Ana Gabriela Reis³ 

[1] Fundação Ezequiel Dias, Belo Horizonte, Brasil;Universidade Federal de Minas Gerais, Belo Horizonte, Brasil;Universidade Federal de São João Del Rei, Divinópolis, Brasil

关键词: validation;    etoposide;    poly(ε;    -;    caprolactone);    intraocular implant.;   

DOI  :  10.1590/S0100-40422012000600032

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A method using HPLC-UV was developed and validated for the determination of etoposide incorporated into polycaprolactone implants. The method was carried out in isocratic mode using a C18 column (250 x 4.6 mm; 5 µm), at 25 ºC, with acetonitrile and acetic acid 4% (70:30) as mobile phase, a flow rate of 2 mL/min, and UV detection at 285 nm. The method was linear (r2 > 0.99) over the range of 5 to 65 µg/mL, precise (RSD < 5%), accurate (recovery of 98.7%), robust, selective regarding excipient of the sample, and had a quantitation limit equal to 1.76 µg/mL. The validated method can be successfully employed for routine quality control analyses.

【 授权许可】

CC BY