Determinação rápida de fármacos básicos em plasma por cromatografia a gás com detector de nitrogênio e fósforo

Química Nova

Lilian Feltraco Lizot²  Luana Letícia Da Silva²  Bárbara Spaniol²  Marina Venzon Antunes²  Rafael Linden²  Rafael Lanaro¹ 

[1] ,Universidade Feevale Instituto de Ciências da Saúde Novo Hamburgo RS ,Brasil

关键词: GC-NPD;    emergency toxicology;    drug screening;   

DOI  :  10.1590/S0100-40422012000600029

来源: SciELO

【 摘 要 】

A simple and fast method for determination of 40 basic drugs in human plasma employing gas-chromatography with nitrogen-phosphorus detection was developed and validated. Drugs were extracted from 800 µL of plasma with 250 µL of butyl acetate at basic pH. Aliquots of the organic extract were directly injected on a column with methylsilicone stationary phase. Total chromatographic run time was 25 min. All compounds were detected in concentrations ranging from therapeutic to toxic levels, with intermediate precision CV% below 11.2 and accuracy in the range of 92-114%.

【 授权许可】

CC BY-NC   
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