Fast determination of basic drugs in plasma by gas chromatography with nitrogen phosphorus detector

Química Nova

Linden, Rafael¹  Silva, Luana Letícia da¹  Lanaro, Rafael¹  Lizot, Lilian Feltraco¹  Antunes, Marina Venzon¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Universidade Feevale, Novo Hamburgo, Brasil¹  Spaniol, Bárbara¹ 

关键词: GC-;    NPD;    emergency toxicology;    drug screening.;   

DOI  :  10.1590/S0100-40422012000600029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A simple and fast method for determination of 40 basic drugs in human plasma employing gas-chromatography with nitrogen-phosphorus detection was developed and validated. Drugs were extracted from 800 µL of plasma with 250 µL of butyl acetate at basic pH. Aliquots of the organic extract were directly injected on a column with methylsilicone stationary phase. Total chromatographic run time was 25 min. All compounds were detected in concentrations ranging from therapeutic to toxic levels, with intermediate precision CV% below 11.2 and accuracy in the range of 92-114%.

【 授权许可】

Unknown