Fast determination of basic drugs in plasma by gas chromatography with nitrogen phosphorus detector

Quimica nova

Lanaro, Rafael¹  Linden, Rafael²  Silva, Luana Letícia da²  Lizot, Lilian Feltraco²  Antunes, Marina Venzon²  Spaniol, Bárbara² 

[1] Universidade Estadual de Campinas, Campinas, Brasil;Universidade Feevale, Novo Hamburgo, Brasil

关键词: GC-;    NPD;    emergency toxicology;    drug screening.;   

DOI  :  10.1590/S0100-40422012000600029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A simple and fast method for determination of 40 basic drugs in human plasma employing gas-chromatography with nitrogen-phosphorus detection was developed and validated. Drugs were extracted from 800 µL of plasma with 250 µL of butyl acetate at basic pH. Aliquots of the organic extract were directly injected on a column with methylsilicone stationary phase. Total chromatographic run time was 25 min. All compounds were detected in concentrations ranging from therapeutic to toxic levels, with intermediate precision CV% below 11.2 and accuracy in the range of 92-114%.

【 授权许可】

CC BY