Immobilization of laccase on hybrid layered double hydroxide

Química Nova

David Isidoro Camacho Córdova²  Rogelio Morales Borges²  Gregorio Guadalupe Carbajal Arizaga¹  Fernando Wypych¹  Nádia Krieger¹ 

[1] ,Universidad de Guadalajara Departamento de Química Guadalajara,México

关键词: laccase;    layered double hydroxide;    immobilization;   

DOI  :  10.1590/S0100-40422009000600025

来源: SciELO

【 摘 要 】

Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.

【 授权许可】

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