Immobilization of laccase on hybrid layered double hydroxide

Quimica nova

Camacho Córdova, David Isidoro¹  Morales Borges, Rogelio¹  Wypych, Fernando²  Krieger, Nádia²  Arizaga, Gregorio Guadalupe Carbajal² 

[1] Universidad de Guadalajara, Guadalajara, México;Universidade Federal do Paraná, Curitiba

关键词: laccase;    layered double hydroxide;    immobilization.;   

DOI  :  10.1590/S0100-40422009000600025

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Crystals of Mg/Allayered double hydroxide were synthesized by alkaline precipitation and treatedin an aqueous solution of glutamic acid. The glutamate ions were not intercalatedinto the interlayer space, but were detected in the material by Fourier transforminfrared spectroscopy, suggesting that only the external surfaces of crystalswere modified with glutamate ions. The resulting hybrid material was testedas a support for immobilization of the enzyme laccase (Myceliophthora thermophila).The immobilized enzyme preparation was characterized by electronic paramagneticresonance spectroscopy and by assays of catalytic activity. The activity ofthe immobilized laccase was 97% of the activity in the free enzyme. Layereddouble hydroxide is a suitable support for use in remediation of soil studies.

【 授权许可】

CC BY