Immobilization of laccase on hybrid layered double hydroxide

Química Nova

Universidade Federal do Paraná, Curitiba¹  Wypych, Fernando¹  Krieger, Nádia¹  Arizaga, Gregorio Guadalupe Carbajal¹  Camacho Córdova, David Isidoro¹  Universidad de Guadalajara, Guadalajara, México¹  Morales Borges, Rogelio¹ 

关键词: laccase;    layered double hydroxide;    immobilization.;   

DOI  :  10.1590/S0100-40422009000600025

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.

【 授权许可】

Unknown