Determinação de fósforo biodisponível em rações de peixes utilizando extração assistida por ultra-som e espectrofotometria no visível

Química Nova

Paula M. De Moraes²  Vanessa R. Loureiro²  Pedro M. Padilha²  Renato De Cássio F. Neves¹  Mayra A. D. Saleh¹  Felipe A. Dos Santos¹  Fábio A. Silva¹ 

[1] ,Universidade Estadual Paulista ‘Julio de Mesquita Filho’ Instituto de Biociências Departamento de Química e BioquímicaBotucatu SP ,Brasil

关键词: ultrasound extraction;    fish nutrition;    phosphorus absorption;   

DOI  :  10.1590/S0100-40422009000400018

来源: SciELO

【 摘 要 】

The aim of the present work was to develop and optimize a method for determination of bioavailable phosphorus in samples of feces and fish feed using ultrasound extraction and subsequent quantification by visible spectrophotometry. Using as extractor solution HNO3 0.50 mol L-1, the great conditions of extraction established were: sample mass - 100 mg, samples granulometry - < 60 µm, sonification time - five cycles of 40 s and ultrasound potency - 136 W. The proposed method was applied in studies of digestibility of this nutrient in different feeds used in diets of juvenile of Nile tilapia.

【 授权许可】

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