Determination of bioavailable phosphorus in fish feeds by ultrasound extraction and visible spectrophotometry

Química Nova

Moraes, Paula M. de¹  Neves, Renato de Cássio F.¹  Santos, Felipe A. dos¹  Padilha, Pedro M.¹  Saleh, Mayra A. D.¹  Universidade Estadual Paulista ‘Julio de Mesquita Filho’, Botucatu, Brasil¹  Loureiro, Vanessa R.¹  Silva, Fábio A.¹ 

关键词: ultrasound extraction;    fish nutrition;    phosphorus absorption.;   

DOI  :  10.1590/S0100-40422009000400018

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of the present work was to develop and optimize a method for determination of bioavailable phosphorus in samples of feces and fish feed using ultrasound extraction and subsequent quantification by visible spectrophotometry. Using as extractor solution HNO3 0.50 mol L-1, the great conditions of extraction established were: sample mass - 100 mg, samples granulometry - < 60 µm, sonification time - five cycles of 40 s and ultrasound potency - 136 W. The proposed method was applied in studies of digestibility of this nutrient in different feeds used in diets of juvenile of Nile tilapia.

【 授权许可】

Unknown