Determination of bioavailable phosphorus in fish feeds by ultrasound extraction and visible spectrophotometry

Quimica nova

Moraes, Paula M. de¹  Neves, Renato de Cássio F.¹  Santos, Felipe A. dos¹  Padilha, Pedro M.¹  Saleh, Mayra A. D.¹  Loureiro, Vanessa R.¹  Silva, Fábio A.¹ 

[1] Universidade Estadual Paulista ‘Julio de Mesquita Filho’, Botucatu, Brasil

关键词: ultrasound extraction;    fish nutrition;    phosphorus absorption.;   

DOI  :  10.1590/S0100-40422009000400018

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of thepresent work was to develop and optimize a method for determination of bioavailablephosphorus in samples of feces and fish feed using ultrasound extraction andsubsequent quantification by visible spectrophotometry. Using as extractor solutionHNO3 0.50 mol L-1, the great conditions of extractionestablished were: sample mass - 100 mg, samples granulometry - < 60 µm,sonification time - five cycles of 40 s and ultrasound potency - 136 W. Theproposed method was applied in studies of digestibility of this nutrient indifferent feeds used in diets of juvenile of Nile tilapia.

【 授权许可】

CC BY