Determinação de fármacos diuréticos em associação por cromatografia em camada delgada e espectrofotometria

Química Nova

Dênia Mendes De Sousa Valladão²  Massao Ionashiro¹  José Zuanon Netto¹ 

[1] ,Universidade do Estado do Mato Grosso Departamento de Engenharia Sinop MT ,Brasil

关键词: thin layer chromatography;    quality control;    diuretics;   

DOI  :  10.1590/S0100-40422008000100009

来源: SciELO

【 摘 要 】

A rapid, sensitive and reliable thin-layer chromatography/spectrophotometry screening procedure was developed for quantitative determination of diuretics associated in pharmaceutical dosage forms. The chromatographic method employed microcrystalline cellulose and butanol : acetic acid : water (4:1:1) or amilic alcohol : ammonium hydroxide 25% (9:1) as mobile phases and detection by U.V. light. The drugs were extracted using a simple procedure and were quantified by U.V. spectrophotometry. Results varied from 97.5 to 102.5% and are similar to those obtained by conventional methods. This method of quantification of diuretics is promising for quality control of drugs.

【 授权许可】

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