Determination of diuretic drugs by thin layer chromatography and spectrophotometry

Química Nova

Universidade do Estado do Mato Grosso, Sinop, Brasil¹  Zuanon Netto, José¹  Valladão, Dênia Mendes de Sousa¹  Instituto de Química de Araraquara, Araraquara, Brasil¹  Ionashiro, Massao¹ 

关键词: thin layer chromatography;    quality control;    diuretics.;   

DOI  :  10.1590/S0100-40422008000100009

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A rapid, sensitive and reliable thin-layer chromatography/spectrophotometry screening procedure was developed for quantitative determination of diuretics associated in pharmaceutical dosage forms. The chromatographic method employed microcrystalline cellulose and butanol : acetic acid : water (4:1:1) or amilic alcohol : ammonium hydroxide 25% (9:1) as mobile phases and detection by U.V. light. The drugs were extracted using a simple procedure and were quantified by U.V. spectrophotometry. Results varied from 97.5 to 102.5% and are similar to those obtained by conventional methods. This method of quantification of diuretics is promising for quality control of drugs.

【 授权许可】

Unknown