Determination of diuretic drugs by thin layer chromatography and spectrophotometry

Quimica nova

Zuanon Netto, José¹  Ionashiro, Massao¹  Valladão, Dênia Mendes de Sousa² 

[1] Instituto de Química de Araraquara, Araraquara, Brasil;Universidade do Estado do Mato Grosso, Sinop, Brasil

关键词: thin layer chromatography;    qualitycontrol;    diuretics.;   

DOI  :  10.1590/S0100-40422008000100009

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A rapid, sensitive and reliable thin-layer chromatography/spectrophotometryscreening procedure was developed for quantitative determination of diureticsassociated in pharmaceutical dosage forms. The chromatographic method employedmicrocrystalline cellulose and butanol : acetic acid : water (4:1:1) or amilicalcohol : ammonium hydroxide 25% (9:1) as mobile phases and detection by U.V.light. The drugs were extracted using a simple procedure and were quantifiedby U.V. spectrophotometry. Results varied from 97.5 to 102.5% and are similarto those obtained by conventional methods. This method of quantification ofdiuretics is promising for quality control of drugs.

【 授权许可】

CC BY