Validação de método por cromatografia líquida de alta eficiência para determinação da lamivudina e zidovudina em comprimidos

Química Nova

Ruy Carlos Ruver Beck¹  Simone Gonçalves Cardoso¹  Margareth Linde Athayd¹  Cristiane Codevilla¹  Fernanda Kreutz De Oliveira¹  Sérgio Luiz Dalmora¹ 

[1] ,Universidade Federal de Santa Maria Centro de Ciências de Saúde Departamento de Farmácia IndustrialSanta Maria RS ,Brasil

关键词: HPLC;    lamivudine;    zidovudine;   

DOI  :  10.1590/S0100-40422007000500033

来源: SciELO

【 摘 要 】

An HPLC method was validated to assay lamivudine and zidovudine combined in tablets. The chromatographic separation was carried out using methanol and acetate buffer pH 6.5 (50:50 v/v) and a RP-18 column, as mobile and stationary phase, respectively. The UV detection was at 270 nm. The method was linear in the range of 24 - 36 µg/mL (lamivudine) and 48 - 72 µg/mL (zidovudine). The recovery (accuracy) ranged from 101.35% to 103.04% and the precision (repeatability and intermediate precision) was less than 2%. The method can be also applied to the quantification of these drugs in the dissolution test of tablets containing both drugs.

【 授权许可】

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