Multivariate spectroscopic determination of the lamivudine-zidovudine association

Journal of the Brazilian Chemical Society

Messerschmidt, Iara¹  Rodrigues, Letícia N. C¹  Cordeiro, Gilcélia A¹  Nagata, Noemi¹  Universidade Federal de São Paulo, Diadema, Brazil¹  Universidade Federal do Paraná, Curitiba, Brazil¹  Peralta-Zamora, Patricio¹ 

关键词: lamivudine;    zidovudine;    electronic spectroscopy;    multivariate calibration;   

DOI  :  10.1590/S0103-50532011000200020

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The multivariate spectroscopic determination of lamivudine-zidovudine associations was carried out by partial least square regression (PLS). This model was developed from 20 synthetic mixtures using mean-centered spectral data acquired from 190 to 350 nm and with 3 latent variables. External validation was performed with 6 synthetic mixtures providing prediction errors close to 1%. Moreover, the analysis of commercial drugs showed good results with prediction errors lower than 10%. The multivariate methodology was validated according to International Conference on Harmonization (ICH) criteria, demonstrating precision, accuracy and robustness within legal requirements.

【 授权许可】

Unknown