Efeito do polietilenoglicol e da albumina na imobilização de lipase microbiana e na catálise em meio orgânico

Química Nova

Cleide Mara Faria Soares²  Maria Helena Andrade Santana²  Gisella M. Zanin¹  Heizir F. De Castro¹ 

[1] ,Universidade Estadual de Campinas Faculdade de Engenharia Química Departamento de Processos BiotecnológicosCampinas SP

关键词: additives;    controlled pore silica;    immobilized lipase;   

DOI  :  10.1590/S0100-40422003000600010

来源: SciELO

【 摘 要 】

Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.

【 授权许可】

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