Effect of poly (ethylene) glycol and albumin on the immobilization of microbial lipase and catalysis and catalysis in organic media

Quimica nova

Castro, Heizir F. de¹  Santana, Maria Helena Andrade²  Soares, Cleide Mara Faria²  Zanin, Gisella M.³ 

[1] Faculdade de Engenharia Química de Lorena, Lorena;Universidade Estadual de Campinas, Campinas;Universidade Estadual de Maringá, Maringá

关键词: additives;    controlled pore silica;    immobilized lipase.;   

DOI  :  10.1590/S0100-40422003000600010

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Poly (ethylene) glycol (PEG) and bovine serumalbumin (BSA), as additive agents, were used to enhance the activity of immobilizedmicrobial lipase in organic solvent. Controlled pore silica (CPS) was selectedas matrix and different immobilization procedures were evaluated: directly lipasebinding on CPS and simultaneous addition of lipase and additive agent on thesame support. The highest coupling yield (59.6%) was attained when the immobilizationprocedure was performed at lipase loading of 150 U/g support in the presenceof PEG-1.500. This immobilized system was used in esterification reactions underrepeated batch cycles and the biocatalyst half-life was found to increase 2.7times when compared with the control.

【 授权许可】

CC BY