Effect of poly (ethylene) glycol and albumin on the immobilization of microbial lipase and catalysis and catalysis in organic media

Química Nova

Santana, Maria Helena Andrade¹  Faculdade de Engenharia Química de Lorena, Lorena¹  Soares, Cleide Mara Faria¹  Zanin, Gisella M.¹  Castro, Heizir F. de¹  Universidade Estadual de Maringá, Maringá¹  Universidade Estadual de Campinas, Campinas¹ 

关键词: additives;    controlled pore silica;    immobilized lipase.;   

DOI  :  10.1590/S0100-40422003000600010

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.

【 授权许可】

Unknown