In vitro organogenesis from internodal segments of adult sweet orange plants

Pesquisa Agropecuária Brasileira

Meire Menezes Bassan²  Francisco De Assis Alves Mourão Filho²  Luzia Yuriko Miyata²  Beatriz Madalena Januzzi Mendes¹ 

[1] ,Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Piracicaba SP ,Brazil

关键词: Citrus sinensis;    adventitious buds;    biotechnology;    plant growth regulators;    Citrus sinensis;    gemas adventícias;    biotecnologia;    reguladores vegetais;   

DOI  :  10.1590/S0100-204X2011000600015

来源: SciELO

【 摘 要 】

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.

【 授权许可】

CC BY   
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