Tissue culture parameters in sweet orange cultivars

Pesquisa Agropecuária Brasileira

Amanda Ferreira Da Silva Mendes²  Luciana Cardoso Cidade²  Gabrielle Novais Manzoli²  Wagner Campos Otoni¹  Walter Dos Santos Soares Filho¹  Marcio Gilberto Cardoso Costa² 

[1] ,Universidade Estadual de Santa Cruz Departamento de Ciências Biológicas Ilhéus BA ,Brazil

关键词: Citrus sinensis;    biotechnology;    gene transfer;    morphogenesis;    regeneration;    Citrus sinensis;    biotecnologia;    transferência gênica;    morfogênese;    regeneração;   

DOI  :  10.1590/S0100-204X2008000800020

来源: SciELO

【 摘 要 】

The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.

【 授权许可】

CC BY   
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