Comparison of Antibacterial Activity in Ethanol Extract and Essential Oil of Citrus sinensis (L.) Peels Obtained by Sohxlet and Distillation Methods

International Fundamentum Science Symposium 2018

自然科学;工业技术

Kusrini, E.^1 ; Mawarni, D.P.^2 ; Mamat, M.^3 ; Prasetyanto, E.A.^4 ; Usman, A.^5

Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok

16424, Indonesia^1

Faculty of Medical, Veteran National Development University of Jakarta, 16514, Indonesia^2

Advanced Nano Materials Group, School of Fundamental Science, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu

21030, Malaysia^3

Faculty of Medicine, Atma Jaya Catholic University of Indonesia, Jakarta

14440, Indonesia^4

Department of Chemistry, Faculty of Science, Universiti Brunei Darussalam, Jalan Tungku Link, Gadong, Negara

BE1410, Brunei^5

关键词: Anti-bacterial activity;    Bioactive compounds;    Citrus sinensis;    Distillation method;    Ethanol extract;    Inhibitory effect;    Soxhlet extraction;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/440/1/012028/pdf
DOI  :  10.1088/1757-899X/440/1/012028

来源: IOP

【 摘 要 】

Ethanol extract and essential oil of Citrus sinensis (L.) peels have been characterized and compared for its antibacterial activity. Ethanol extract of C. sinensis (L.) peels was obtained by Soxhlet extraction method in ethanol with purity of 96% and the essential oil was obtained by distillation method for 5 hours at 100°C. The antibacterial activities of the extracts were investigated against Staphylococcus aureus. The experimental results showed that the ethanol extract yielded 29.50% (w/w), with total flavonoid compounds of 4.74 mg/g and polyphenolic of 20.54 mg/g, while the water extract (essential oil) yielded 6.80% (w/w) with total polyphenolic compounds of 0.65 mg/g. The flavonoid compound was not observed in the essential oil obtained from distillation method. The bioactive compounds in the ethanol extract obtained by Soxhlet extraction are larger than those found in the essential oil obtained by distillation method. The ethanol extract showed stronger growth inhibitory effect of Staphylococcus aureus with zone inhibition diameter of 18 mm, whereas the essential oil and the commercial d-limonene extract had similar zone inhibition diameter of 15 mm.