Bacterial spot and early blight biocontrol by epiphytic bacteria in tomato plants

Pesquisa Agropecuária Brasileira

Roberto Lanna Filho²  Reginaldo Da Silva Romeiro¹  Eduardo Alves² 

[1] ,Universidade Federal de Lavras Departamento de Fitopatologia Lavras MG ,Brazil

关键词: Alternaria solani;    Bacillus pumilus;    Paenibacillus macerans;    Xanthomonas vesicatoria;    biological control;    epiphytic bacteria;    Alternaria solani;    Bacillus pumilus;    Paenibacillus macerans;    Xanthomonas vesicatoria;    controle biológico;    bactéria epifítica;   

DOI  :  10.1590/S0100-204X2010001200007

来源: SciELO

【 摘 要 】

The objective of this work was to evaluate in vitro and in vivo biocontrol of bacterial spot (Xanthomonas vesicatoria) and early blight (Alternaria solani) by the epiphytic bacteria Paenibacillus macerans and Bacillus pumilus. Tomato plants were previously sprayed with epiphytic bacteria, benzalkonium chloride and PBS buffer and, after four days, they were inoculated with A. solani and X. vesicatoria. To determine the phytopathogenic bacteria population, leaflet samples were collected from each treatment every 24 hours, for seven days, and plated on semi-selective medium. The effect of epiphytic bacteria over phytopathogens was performed by the antibiosis test and antagonistic activity measured by inhibition zone diameter. The epiphytic and benzalkonium chloride drastically reduced the severity of early blight and bacterial spot in comparison to the control (PBS). In detached leaflets, the epiphytic bacteria reduced in 70% the number of phytopathogenic bacteria cells in the phylloplane. The antibiosis test showed that the epiphytic bacteria efficiently inhibit the phytopathogens growth. In all the bioassays, the epiphytic bacteria protect tomato plants against the phytopathogens

【 授权许可】

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