Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area

Memórias do Instituto Oswaldo Cruz

Hai-mo Shen¹  Shen-bo Chen¹  Yue Wang¹  Jun-hu Chen¹ 

关键词: Plasmodium vivax;    genome;    vir;   

DOI  :  10.1590/0074-02760150216

来源: SciELO

【 摘 要 】

Currently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we report the first genome sequencing of a P. vivaxisolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivaxSalvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novoassembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known virgenes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research onP. vivaxpopulation genetics.

【 授权许可】

CC BY   
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