The presence of Helicobacter pylori in the liver depends on the Th1, Th17 and Treg cytokine profile of the patient

Memórias do Instituto Oswaldo Cruz

Luciana Diniz Silva²  Andreia Maria Camargos Rocha²  Gifone Aguiar Rocha²  Sílvia Beleza De Moura¹  Márcia Maria Negreiros Pinto Rocha²  Renato Dani¹  Fabrício Freire De Melo²  Juliana Becattini Guerra²  Lúcia Porto Fonseca De Castro¹  Guilherme Santiago Mendes¹  Teresa Cristina De Abreu Ferrari¹  Agnaldo Soares Lima¹  Dulciene Maria Magalhães Queiroz² 

[1] ,Universidade Federal de Minas Gerais Instituto de Ciências Biológicas Laboratório de Pesquisa em BacteriologiaBelo Horizonte MG ,Brasil

关键词: Helicobacter pylori;    liver diseases;    IL-10;    IL-17;    IFN-γ;   

DOI  :  10.1590/S0074-02762011000600016

来源: SciELO

【 摘 要 】

The hypothesis that Helicobactermight be a risk factor for human liver diseases has arisen after the detection of Helicobacter DNA in hepatic tissue of patients with hepatobiliary diseases. Nevertheless, no explanation that justifies the presence of the bacterium in the human liver has been proposed. We evaluated the presence of Helicobacterin the liver of patients with hepatic diseases of different aetiologies. We prospectively evaluated 147 patients (106 with primary hepatic diseases and 41 with hepatic metastatic tumours) and 20 liver donors as controls. Helicobacter species were investigated in the liver by culture and specific 16S rDNA nested-polymerase chain reaction followed by sequencing. Serum and hepatic levels of representative cytokines of T regulatory cell, T helper (Th)1 and Th17 cell lineages were determined using enzyme linked immunosorbent assay. The data were evaluated using logistic models. Detection of Helicobacter pylori DNA in the liver was independently associated with hepatitis B virus/hepatitis C virus, pancreatic carcinoma and a cytokine pattern characterised by high interleukin (IL)-10, low/absent interferon-γ and decreased IL-17A concentrations (p < 10-3). The bacterial DNA was never detected in the liver of patients with alcoholic cirrhosis and autoimmune hepatitis that are associated with Th1/Th17 polarisation. H. pylori may be observed in the liver of patients with certain hepatic and pancreatic diseases, but this might depend on the patient cytokine profile.

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