Antimicrobial resistance profiles and genetic characterisation of macrolide resistant isolates of Streptococcus agalactiae

Memórias do Instituto Oswaldo Cruz

Priscila Am Nakamura¹  Rôde Beatriz B Schuab¹  Felipe Pg Neves¹  Cláudio Fa Pereira¹  Geraldo R De Paula²  Rosana R Barros¹ 

[1] ,Instituto Biomédico Departamento de Microbiologia e Parasitologia

关键词: Streptococcus agalactiae;    antimicrobial resistance;    erythromycin resistance genes;   

DOI  :  10.1590/S0074-02762011000200001

来源: SciELO

【 摘 要 】

In this study, 100 clinical isolates of Streptococcus agalactiae recovered from genitourinary tract specimens of non-pregnant individuals living in Rio de Janeiro were submitted for antimicrobial susceptibility testing, detection of macrolide resistance genes and evaluation of the genetic diversity of erythromycin-resistant isolates. By agar diffusion method, all isolates were susceptible to ceftazidime, penicillin and vancomycin. Isolates were resistant to levofloxacin (1%), clindamycin (5%), erythromycin (11%) and tetracycline (83%) and were intermediated to erythromycin (4%) and tetracycline (6%). Erythromycin-resistant and intermediated isolates presented the following phenotypes: M (n = 3), constitutive macrolide-lincosamide-streptogramin B (MLS B, n = 5) and inductive MLS B (n = 7). Determinants of macrolide resistance genes, erm and mef, were detected in isolates presenting MLS B and M phenotypes, respectively. Randomly amplified polymorphic DNA profiles of erythromycin-resistant isolates were clustered into two major groups of similarity.

【 授权许可】

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