Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil

Memórias do Instituto Oswaldo Cruz

Rv Laurentino²  Igl Lopes²  Vn Azevedo²  Lfa Machado²  Mrc Moreira¹  L Lobato¹  Mog Ishak²  R Ishak²  Acr Vallinoto² 

[1] ,Universidade Federal do Pará Centro de Ciências Biológicas Departamento de PatologiaBelém PA ,Brasil

关键词: human T-cell lymphotropic virus;    human immunodeficiency virus 1;    retrovirus;    coinfection;    molecular epidemiology;   

DOI  :  10.1590/S0074-02762005000400006

来源: SciELO

【 摘 要 】

The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.

【 授权许可】

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