Standardization of in-house polymerase chain reaction for the identification of Mycobacterium tuberculosis at the reference tropical disease hospital in the State of Goiás, Brazil

Memórias do Instituto Oswaldo Cruz

Márcia Alves Vasconcelos Rodrigues²  Álvaro Bisol Serafini²  Marieta De Souza Pereira²  Thathiane Dias Da Silva¹  Marcelo Fouad Rabahi¹  Suely Lemes De Alves¹  André Kipnis² 

[1] ,Universidade Federal de Goiás Instituto de Patologia Tropical e Saúde Pública Departamento de MicrobiologiaGoiânia GO ,Brasil

关键词: Mycobacterium tuberculosis;    polymerase chain reaction;    tuberculosis;    culture;    smear;   

DOI  :  10.1590/S0074-02762004000400012

来源: SciELO

【 摘 要 】

This study compares smear, growth in Lowenstein-Jensen medium, and in-house polymerase chain reaction (PCR) techniques for the detection of Mycobacterium tuberculosis. A total of 72 specimens from 72 patients with clinical symptoms of tuberculosis, including 70 sputum and two bronchial aspirate samples, were tested in parallel by smear, culture, and in-house PCR techniques. From these, 48 (66.6%) were negative by the 3 methods, 2 (2.8%) were smear positive and negative by culture and in-house PCR, 11 (15.3%) were both smear and culture negative, and in-house PCR positive, 7 (9.7%) were positive by the 3 methods, 2 (2.8%) were positive by smear and culture, and negative by PCR, 2 (2.8%) were positive by culture and PCR, but smear negative. After the resolution of discrepancies in PCR results, the sensitivity and specificity for in-house PCR technique to M. tuberculosis relative to the culture, were 81.8% and 81.9%, respectively. These results confirm that this method, in-house PCR, may be a sensitive and specific technique for M. tuberculosis detection, occurring in both positive and negative smear and negative cultures.

【 授权许可】

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