Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)

Memórias do Instituto Oswaldo Cruz

Afc Nassar²  S Miyashiro²  Cg Oliveira¹  Wa Pacheco²  Ra Ogata¹ 

[1] ,Instituto BiológicoSão Paulo SP ,Brasil

关键词: tuberculosis;    bovine;    Mycobacterium bovis;    intradermal tuberculin test;    isolation;    polymerase chain reaction;   

DOI  :  10.1590/S0074-02762007005000073

来源: SciELO

【 摘 要 】

Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) and the other for differentiating M. tuberculosis from M. bovis. Twenty seven samples (64.3%) from 18 animals (60%) were positive for mycobacteria by culture, including samples from 15 retrofaryngeal lymphnodes (55.5%), 9 prescapular lymphnodes (33.3%), 2 lungs (7.4%), and 1 liver (3.7%). All isolated colonies were confirmed by PCR to contain MTBC organisms, and were identified as M. bovis by the same methodology.

【 授权许可】

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