Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

Memórias do Instituto Oswaldo Cruz

Claudia C Rubiano¹  Moises Wasserman¹ 

关键词: Plasmodium;    malaria;    telomerase;    assay;    nonradioactive;   

DOI  :  10.1590/S0074-02762003000500018

来源: SciELO

【 摘 要 】

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10² to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

【 授权许可】

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